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  1. Bring the DNA plate along with your PCR plate to the barcode scanner in the lab.
  2. Go to LIMS http://131.104.104.75:8080/Lims.Web/
  3. Under Stage Selection choose PCR Prep.
  4. User Barcode: Scan in your card. Start plate: scan in the barcode on the extracted DNA. End plate: put a barcode on your PCR plate and scan it in.
  5. Choose the Protocol you used (eg. If you used primers MLepF/LepR, that is what you choose from the list). Press Submit. The plate barcodes will now be gone if it worked.
  6. Go back to the LIMS home page. Under Stage Selection choose PCR Thermocycling.
  7. User Barcode: Scan in your card. Plate barcode: scan in the barcode on your PCR plate. Choose the Protocol (this is which program you ran the reaction on in the thermocycler, eg. COIFAST).
  8. Press Submit.

Return to PCR Procedure.

Continue to E-gel PCR Product Check.

Updated April 27 2009