1. Make sure that the Matrix Box and the lysis plate are aligned the same way (so that if A1 is in the top left corner of the Box, it is also in the top left corner of the plate). Check it again, just to make sure. Write “Lysed” on the Matrix Box.
  2. Get four jars. In the first one, label it “Eliminase” put about 2.5 cm high amount of Eliminase.
  3. In the next three jars, label them “Wash 1”, “Wash 2”, and “Wash 3” and fill them all with 3 cm or more of deionized water.
  4. There is a small tool that looks like an Allen key that is used to open the top on the tubes that come in the Matrix Box.
  5. Remove the first cap-strip row (1) from the lysis plate, and place it on a Kim Wipe. Take out the first tube (H1), and place it in a tube rack. Open the top.
  6. Dip your tools into the Eliminase, and shake them a bit. Remove them and wipe off the excess liquid.
  7. Then dip them and shake them slightly in Wash 1, Wash 2, and finally in Wash 3. Dry them off.
  8. Do this before your first sample, between samples and after your last one.
  9. Pull out the tissue and remove a small piece of it (about 1mm3 for fish tissue) and place it in the correct well in the lysis plate. Make sure to sterilize the forceps between each sample with ethanol and flame. Put each tube back in its proper place in the Matrix Box before you get the next one.
  10. Put the cap-strips back on as you go. The caps come in rows of eight, and one tab says A and the other says G, this corresponds to the A and G on the plate.
  11. Put the Lysis plate onto LIMS, and then place it in the incubator in BIO at 56 oC over night (for at least 12 hours, but at the very least 8 hours).

Next you have to extract the DNA with either the Robotic DNA Extraction in BIO or with Manual Extraction.

Return to Barcoding in the Hanner Lab Wiki.

Updated April 27 2009